2 mercaptoethanol (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher 2 mercaptoethanol
2 Mercaptoethanol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2+mercaptoethanol/2-Mercaptoethanol%2C+98%2B%25/pmc13170521-241-31-34
Average 99 stars, based on 1 article reviews

2 mercaptoethanol - by Bioz Stars, 2026-09

99/100 stars

Images

Cell Culture:

Article Title: Innovative photodynamic therapy using rose bengal for the treatment of human melanoma
Article Snippet: PBMCs were isolated by density gradient centrifugation of the blood using a lymphocyte separation medium (Eurobio, Les Ullis, France) and 50 mL Leucosep tubes (Greiner Bio One, Courtaboeuf, France). .. In round-bottom 96-well plate (Corning, Somerville, MA, USA) 100,000 PBMCs were cultured in ML10 medium composed of RPMI 1640 medium supplemented with HEPES (25 mM), non-essential amino acids (MEM 1 x ), sodium pyruvate (1 mM), 2-mercaptoethanol (50 μM), gentamicin (10 μg/mL) (Thermo Fisher Scientific, Waltham, MA, USA), and 10% of SAB (Human AB serum). .. HBL and LND cells were seeded in 96 well culture plates (Corning, Somerville, MA, USA) at the concentration of 15,000 cells per wells and incubate for 24 h. Then cells were treated with increasing doses of RB (0, 5, 10, 25, 50, 75, 100, and 400 μM) and during several incubation time points (0, 0.5, 1, 2, 3, and 4 h).

Article Title: Comparative Assessment of Cartilage Quality in Human Induced Chondrocytes (hiCHOs) and Primary Articular Chondrocytes (hACs) Following Fibronectin-Based Selection.
Article Snippet: .. At every passage of each condition, 2.5 x 105 hiCPCs were harvested and centrifuged for 5 min at 1640 rpm in order to form aggregates that were subsequently cultured and maintained in 3D suspension culture in 15 mL tubes in chondrogenic differentiation (CD) medium containing Dulbecco’s modified Eagle’s medium/F12 (Gibco), supplemented with 1% ITS+, 55 μM 2-mercaptoethanol (Gibco), 1 % non-essential amino acids (Gibco), 0.5 % P/S, L-ascorbate-2-phosphate (50 μg/ml; Sigma-Aldrich), L-proline (40 μg/ml; Sigma-Aldrich), ML329 (1 μM; CSNpharm), C59 (1 μM; Tocris), and transforming growth factor–β1 (10 ng/ml; Peprotech) for up to 28 days to obtain human induced chondrocytes (hiCHOs) while refreshing medium every 3 to 4 days. ..

In Vitro:

Article Title: cGAS/STING sensing in dendritic cells discriminates between daptomycin sensitive and resistant Staphylococcus aureus clinical isolates
Article Snippet: .. MutuDC (line 2114, obtained from Tom Brodnicki at St. Vincent’s Hospital Melbourne) were seeded at 1.5 × 10 6 cells per mL in complete media (CM; Gibco RPMI-1640 GlutaMAX, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal calf serum (FCS; In Vitro Technologies, Noble Park, Melbourne, VIC, Australia), 100 μM 2-mercaptoethanol (Thermo Fisher Scientific) and 0.01% (w/v) penicillin/streptomycin (Thermo Fisher Scientific) adjusted to mouse tonicity (308 mOsm/L), and maintained at 37°C 10% CO 2 , with passaging at 80–90% confluency. ..

Passaging:

Suspension:

Modification:

Single Cell:

Article Title: Image-enabled cell sorting permits prospective isolation of primitive hematopoietic stem cell subsets with distinct functional potential.
Article Snippet: .. Single-cell division kinetics assays Single ESLAM HSCs were sorted into round-bottom 96-well plates (Falcon), with each well containing StemSpan SFEM (StemCell Technologies, cat no. 09650), supplemented with 1% Penicillin/Streptomycin (Gibco), 1% L-glutamine (Gibco), 0.2% 2-mercaptoethanol (2-ME, Gibco), 300 ng/mL Stem Cell Factor (SCF) and 20 ng/mL Interleukin 11 (IL-11) (Biotechne). ..

Review